(157c) Integrating Bisphenol a Degradation Function into Shewanella Oneidensis

Bisphenol A (BPA) is a major environmental endocrine-disrupting compound and increases concerns about its estrogenic and genotoxic effects on humans. More than one million pounds of BPA are discharged into the environment and most BPA emissions are to the aqueous environment. Also, BPA can affect the growth, reproduction, and development of aquatic organisms. Therefore, removing BPA during water treatment is critical to protect the environment as well as to mitigate human health risk. Biodegradation by microorganisms may be the most efficient method as microorganisms degrade BPA for use as a carbon and energy source. In this study, we develop Shewanella oneidensis MR1 as an efficient BPA degrader while enhancing electron transfer of this microorganisms by forming biofilms in bioelectrochemical systems. First, BPA biodegradation by BisdA (ferredoxin), BisdB (cytochrome P450) from Sphingomonas bisphenolicum AO1 was validated in Escherichia coli BL21 (DE3) Star strain. Our results indicated that BPA was completely degraded within 5 h, measured by LC-MS/MS. Next, we transferred those genes into S. oneidensis MR1 and turned their expression. BPA degradation by S. oneidensis strain and its biofilm formation to form bioelectrochemical system will be discussed. Establishing a BPA degradation pathway coupled with bioelectrochemical systems may be a promising strategy to efficiently remove BPA from wastewater.