(292f) Photo-Crosslinked Nanogel Via Nanoreactor for Therapeutic Proteins Delivery (Award Session)

Nanotechnology-based drug delivery systems use a variety of nanoparticle fabrication methods to formulate the different types of delivery formula. The nano-delivery systems provide the enhanced membrane permeability of the drugs because the nanoscale delivery formula allows the maximum surface area of a particle per unit of mass.

In this study, the polymeric nano-hydrogel (Nanogel) was formulated to deliver therapeutic proteins. The material biocompatibility and delivery efficiency were first taken into consideration to make an effective delivery carrier. Nanogel was synthesized by a process of confining hydrophilic biopolymer in a water-in-oil nanoreactor followed by photopolymerization and purification. This system enhanced the penetration of fluorescent-conjugated bovine serum albumin into a biological membrane indicating its suitability for transdermal drug delivery [1]. The modified nanogel was formulated by using fish gelatin methacrylate to enhance the drug diffusion at the target location without significant cytotoxicity [2]. For testing delivery efficiency and therapeutic effect, a model therapeutic protein was synthesized using a cell-free system described in the previous work [3]. The human skin protein was then encapsulated in the drug carrier and tested on the skin membrane model. Nanogel system showed great potential in transdermal drug delivery of protein therapeutics.

[1] Macromol Res 24 (12), 1115

[2] Mar Drugs 17 (4), 246

[3] Method Protoc 2 (3), 68