(292h) Understanding Corona Exchange Dynamics on Carbon Nanotubes with Multiplexed Fluorescence Monitoring (Award Session)
AIChE Annual Meeting
2020
2020 Virtual AIChE Annual Meeting
Nanoscale Science and Engineering Forum
Bionanotechnology Graduate Student Award Session
Tuesday, November 17, 2020 - 9:45am to 10:00am
We present an assay to study the corona exchange dynamics between solution-phase and corona-phase polymers on SWCNTs.3 This assay exploits the quenching property of fluorophores proximal to the SWCNT surface to monitor ligand binding and unbinding events. Real-time tracking of polymer adsorption and desorption events on the SWCNT surface are conducted with systematic variation of the molecular entities and solution conditions, with a specific focus on DNA (as the sensing moiety) and protein (as the biofouling agent). Binding profiles are extracted from the experimental assay and used to inform a kinetic model of the system. The work presented herein develops an understanding of the fundamental corona exchange mechanism and provides insight into performance of these designed SWCNT-based systems in biologically relevant, protein-rich conditions.
References
- Demirer, G. S. et al. High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants. Nature Nanotechnology 1 (2019) doi:10.1038/s41565-019-0382-5.
- Beyene, A. G. et al. Imaging striatal dopamine release using a nongenetically encoded near infrared fluorescent catecholamine nanosensor. Science Advances 5, eaaw3108 (2019).
- Pinals, R. L., Yang, D., Lui, A., Cao, W. & Landry, M. P. Corona Exchange Dynamics on Carbon Nanotubes by Multiplexed Fluorescence Monitoring. J. Am. Chem. Soc. (2019) doi:10.1021/jacs.9b09617.