(53b) Toward Understanding of Adsorption of Platinum Metal-Binding Peptide and Its Potential Applications

Metal-binding peptides have become more useful for the development of advanced materials (e.g., catalyst synthesis, drug delivery, and biosensing). Platinum-binding peptides have been utilized in the synthesis of platinum nanoscrystals, one-dimensional platinum nanostructures, and thin-film electrodes. However, a systematic characterization of their non-covalent affinity to platinum is still elusive. Many speculations on how the metal-binding peptide selectively recognizes the surface can be found among the literature. In this work, we investigated the adsorption behavior of a platinum-binding peptide using sensitive mass measurements coupled with binding kinetics modeling. We further investigated the adsorption of the peptide on different red-ox states of a platinum surface using electrochemistry. The preliminary results illustrate the importance of the state of the metal surface, and thus is an important design consideration when using this metal-binding peptide. We have also leveraged this finding to develop a new method for non-chromatographic protein purification. Overall, the approaches used in this study can help clarify how metal-binding peptides are able to selectively recognize surfaces and aid in understanding of the interfacial interactions occurring.