(160z) Interaction Study of Myoglobin and Chlorpyrifos

Long term exposure of pesticides residues on human system is quiet alarming and there is crucial need to keep a check on the amount of pesticides we consumes unknowingly. To solve this issue we need to have a user friendly sensor for detection of toxic chemicals as it affects the metabolism of body. To achieve this crucial target we first need to have efficient analysis of interaction of pesticides with protein which has probability to be used for sensor fabrication. In the present study, we are analyzing the interaction of protein myoglobin and chlorpyrifos, an organophosphate pesticide. Interaction study is carried out both in bulk solution and on surface using isothermal titration calorimeter (ITC) and quartz crystal microbalance (QCM) respectively. The activity of protein is also studied on modified silica surfaces. The result shows maximum activity of protein on bare silica surface as compared to modified surfaces. The ITC results show significant binding of myoglobin with chlorpyrifos in solution and the results shows best fit to set of independent binding model. QCM analysis gives real time adsorption of myoglobin on bare silica surface and subsequently pesticide is adsorbed. The analysis reveals monolayer adsorption of protein on silica surface while it is multilayer for pesticide. Overall the results can be concluded in significant binding of myoglobin and pesticide. This study will be quiet useful to check the effect of pesticide on blood as well as to fabricate a more efficient and selective protein based biosensor for pesticide detection which is most commonly used in the consumable product.

Keywords: Myoglobin, chlorpyrifos, isothermal titration calorimeter, quartz crystal microbalance.

References

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