(179a) Enzyme-Based Composites for Next Generation Gas Sensing Platforms.

Enzymes are biocatalysts that maintain biological systems functionality; synthetic isolation and implementation of enzymes is widely utilized in various fields such as biosensors, pharmaceuticals, biofuel, food and detergents industries, platforms for decontamination, or gas sensors applications. In such fields, enzyme-based conjugates have been created through techniques such as encapsulation, physical or covalent binding or entrapment, with the variety of the binding partners influencing both the conditions of the immobilization processes as well as the long-term operational stability and systems functionality or overall viability. Our research aims to design the next generation of enzyme-based platforms to be used in gas sensors applications. We explored solvothermal and co-precipitation methods to obtain enzyme-based metallic complexes and to create stabilizing microenvironments for catalytic processes associated with gas adsorption, all facilitated by organic and inorganic compounds integration. Enzyme-based metallic complexes morphology, surface area to mass ratios, porosity, chemical and structural structure were assessed through a variety of techniques consisting of scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) spectroscopy, Brunauer–Emmett–Teller (BET), X-ray diffraction (XRD), Fourier transform infrared (FTIR), and X-ray photoelectron (XPS) spectroscopy, respectively. Gas sensing of carbon dioxide (CO2), an industrial setting product and a by-product of anthropogenic activities, was assessed because of its role as a majority of greenhouse gas emission that could potentially harm living species through heat-related illness, thermal absorption, climate change and ocean water feedback. Our analysis present concrete steps for customizable yet affordable synthesis of gas sensing and adsorption platforms based on green technologies resulted from enzyme implementation while also offering discussion on how kinetic coordination of metallic compounds lead to active centers with porous surface formation to allow for increased gas sensing performance to be fine-tuned at interfaces.