(210e) Rapid Semiconductor Sensor System for Cerebral Spinal Fluid Detection Using Disposable Biosensor Strips

Cerebral spinal fluid (CSF) is an extracellular fluid surrounding the brain and spinal cord for nutrition transport and physical buffer. The leakage of cerebral spinal fluid is of crucial clinical concern with respect to the possibility of potentially fatal infections. Current test method for beta-2-transferrin (B2T), a biomarker for endogenous protein only present in CSF, requires hours of turnaround time in centralized laboratory and can potentially delay the diagnosis and timely treatment. Semiconductor based sensor offers the unique advantage of providing fast detection as well as possibilities of modular packaging for point-of-care use. Si metal oxide semiconductor field effect transistor (MOSFET) was implemented for signal amplification purpose, instead of expensive high electron mobility transistor (HEMT). The gate electrode of the transistor is externally connected to a biofunctionalized sensing strip with beta-2-transferrin (B2T) monoclonal antibodies (mAb). Compare to previous generation of sensor that requires complex fabrication steps on glass slides, this work presents the use of factory-premade plastic sensor for cost reduction, improved production throughput, while achieving high sensitivity. With this sensor system, detection of cerebrospinal fluid can be achieved range from 0.1ng/mL to 10mg/mL with one time use sensor strips biofunctionalized with B2T mAb, as well as detection time of less than 1 second. Immediate feedback of presence of CSF leakage via medical sensor lessened the turnaround time compared to traditional laboratory test, and this life-saving technology can be potentially implemented in detection of board range of biomarkers of interest.