(353e) Bioactive Bacterial Cellulose Membranes Loaded with Frutalin Lectin (Artocarpus incisa L.)

The development of bioactive dressing based on bacterial cellulose (BC) loaded with active compounds can improve the treatment of skin wounds. The better experimental conditions of lectin yield into BC matrix were addressed in this study. BC was modified by sodium periodate (OxBC), inserting two aldehyde groups in the polymer chain, increasing the hydrophilicity and increase porosity. This chemical modification was performed to increase the interaction with frutaline hemagglutinating lectin (Artocarpus Incisa L.) improve the skin healing process. The lectin was purified by affinity chromatography, dialysis, and electrophoresis. The lectin activity was confirmed by hemagglutination assay. The cytotoxicity properties of raw frutalin and it loaded on BC were measured using L929 cells (rat fibroblasts). The frutalin/BC or frutalin/OxBC yields were measured according to an experimental design. The independent parameters: temperature (5, 25, and 45 ºC), time (6, 15, and 24 h) of immobilization and frutalin concentration (40, 80, and 120 μg/mL). The protein adsorbed on the membranes was quantified by the Bradford method. A linear regression model for the immobilization yield was developed to describe the significant parameters and their interaction. The purified frutaline did not show any cytotoxicity to L929 cells, in the concentration range of 20-200 μg/mL. The highest yield was obtained at the lowest frutalin concentration and temperature with higher time. This is a promising result depicting that low frutalin concentration can maximize the protein interaction being a good option for skin healing.