(432b) Detection of 3D Position and Orientation of Microparticles Using Holographic Microscopy and Image Moment Techniques
AIChE Annual Meeting
2021
2021 Annual Meeting
Engineering Sciences and Fundamentals
Microfluidic and Microscale Flows: Separations and Particulates
Wednesday, November 10, 2021 - 8:15am to 8:30am
In this work, we developed an imaging method based on single beam in-line digital holographic microscopy (DHM). This methodology utilizes the image moment information to provide a quick, direct measurement of the center of mass and the in-plane/out-of-plane orientations of a non-spherical microparticle in 3-D space. We verified the results are in good agreement with measurements of static images of ellipsoidal particles from brightfield microscopy. We further demonstrate its application in analyzing the migration and orientation trajectory of spheroidal microparticles in a viscoelastic fluid. We also demonstrate the ability of the method to resolve the swimming trajectories of E.Coli cells within an aqueous medium. We further comment on the possible applications of this methodology in various research fields such as suspension rheology, microrheology and microbiology.