(657a) Flexible Hydrogel-Based Biosensor Fabricated Via Direct Laser Writing for Detection of Cortisol

Wearable sensors have attracted much attention thanks to their promising applications in personalized medicine through remote monitoring of physiological parameters. Cortisol is a biomarker for numerous diseases and is important for blood pressure regulation, glucose levels and metabolism. This study is to create a flexible, wearable biosensor to detect cortisol in body fluid by skin contact with the sensor. A laser-assisted processing technique was used to produce laser-induced graphene (LIG) by direct laser writing with a carbon dioxide laser on carbon materials. This LIG substrate was transferred to an elastomeric substrate (PDMS). For non-invasive extraction of biomarkers, a hydrogel layer was incorporated onto the electrode surface. A cortisol antibody was immobilized on the working electrode via surface chemistry. Both cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the modified electrode and perform detection of cortisol. Specifically, the characterizations were carried out in terms of detection limit, selectivity, sensitivity, response time, and linear range. Using 3D printed wristbands and wireless communication devices, it may be possible to monitor personnel in real-time during any sort of situations including long-term exposure to space environments and solid contact during physical activity.