Creation of Shear Flow Wash System for Cellular Micropatterning with Biotin-Streptavidin

In the medical industry, organs and tissue are continuously in demand to maintain the health of patients. There has been a rise in the research and production of artificial tissues and organs to generate resources to meet the necessary demands. The artificial tissues maintain regenerative properties through the use of cells placed on the construct. Surface engineering is used to intentionally place cells in a biomimetic pattern on artificial tissue constructs. Cells were placed onto a substrate by chemically altering the surface with strong binding forces like biotin and streptavidin. These molecules create a specific region for cells with biotin molecules on the surface to bind and generate a desired pattern. Through the use of a parallel plate flow chamber, the substrate with the generated cellular micropattern can be rinsed to remove any cells remaining on the substrate that is not bound to the patterned regions. Using a flow chamber and 25 ml/min flow rate with an exposure time of 15 seconds a clear cellular micropattern can be generated.