(165e) A Comparative Assessment of the Response of Primary and Metastatic Ovarian Cancer Cells to Cisplatin in 3D Models of Various Structural and Biochemical Configurations
AIChE Annual Meeting
2022
2022 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Poster Session: Engineering Fundamentals in Life Science
Monday, November 14, 2022 - 3:30pm to 5:00pm
Herein, we report, for the first time, a comprehensive comparative study of three different 3D in vitro platforms, namely (i) spheroids, (ii) synthetic hydrogels of various chemical configurations and (iii) polymeric scaffolds of various Extracellular Matrix (ECM) coating on the cell growth and response to the chemotherapeutic (Cisplatin) for ovary derived (A2780) and metastatic (SK-OV-3) EOC cell lines.
METHODS: Spheroids of A2780 (primary ovarian cancer) and SK-OV-3 (metastatic ovarian cancer) cell lines were fabricated using specialized 96 well round bottom plates, provided by faCellitate (Manheim, Germany). Synthetic PeptiGels (Manchester BIOGEL, UK) were used as per manufacturerâs instructions for the hydrogel based culture while polyurethane (PU) scaffold assisted 3D cultures was carried out as per our previously published protocol4,5,6,7. The 3D cultures were maintained and monitored for different time periods (10â28 days) depending on the culture platform type. Feasibility of using these models for assessment of chemotherapeutic agent (Cisplatin) was also carried out. Various in situ assays for monitoring the cell viability, cellular apoptosis and spatial organisation were performed using advanced CLSM microscopy
RESULTS & CONCLUSION: We report that all three 3D models can support the growth of EOC, but for different time periods (varying from 7 days to 4 weeks). We have seen that chemoresistance to Cisplatin, in vitro, observed especially for metastatic EOC cells, is platform dependent both in terms of structural as well as in terms of biochemical composition of the model/platform. Our study highlights the selection of the appropriate/best 3D in vitro model depends on the cell type, experimental time period and experimental question being asked as different models are appropriate even when studying. Our study highlights that the selection of a 3D in vitro platform depends on (i) the planned experimental/assessment time period, (ii) the type of cell to be studied, (iii) the site of cell origin in vivo and (iv) the question that needs to be answered 8.
ACKNOWLEDGMENT: The project was supported financially by the 3DBioNet (UKRI). E.V is grateful to the Royal Academy of Engineering for an Industrial Fellowship and to the Medical Research Council UK for a New Investigator Research Grant (MR/V028553/1), which also financially supports P.G.
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