(173i) Study on Growth and Metabolite Trend Comparison of Staphylococcus Aureus and Pseudomonas Aeruginosa in Monoculture and Co-Culture System

One of the most prevalent types of multi-microbial infection occurs in chronic wounds associated with diabetes, cystic fibrosis, and venous disease. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most common bacteria isolated from chronic wounds and are frequently found together, which implies they possess synergistic interactions in the wound environment. Multi-species infections are more virulent and often result in worse patient outcomes than single microbe infections. The microbes often express a biofilm phenotype which has strong tolerance to numerous interventions such as antibiotics. The goal of this project is to understand microbes interaction, and mechanisms in planktonic culture during monoculture and co-culture condition. Our initial investigation revealed that the co-culture system showed 277% increase in growth rate and 138% more biomass production comparing to their monoculture. This indicate that co-culturing system showed growth benefit when these are coexist in growth media. Also their metabolic trend reveals that syntrophy effect takes place in co-culture system by different preference on primary carbon source. It led more stable in lower buffering condition in co-culture system. From this investigation, we will have better understanding how microbes behave in co-culture conditions and the results will be beneficial to find the better approach to treat chronic wound infections.