(621c) A New Strategy of in-Situ Enzyme Immobilization Based on Self-Assembly Hydrogel for Continuous-Flow Biocatalysis for the Synthesis of Chiral Alcohols

Biocatalytic synthesis of chiral alcohols has the unique advantages of green, mild and high selectivity. The reuse of catalysts and the elimination of inhibitory effects depend on the development of immobilization technology and continuous flow process. Traditional immobilization methods are difficult to achieve in both aspects of activity and stability. For the first time, we found a new immobilization strategy based on supramolecular enzyme self-assembly to form hydrogels, which used to catalyze the synthesis of chiral alcohols with a unit activity of six times higher than that of free enzymes and good stability (>95% activity retained after 5 reuses). Moreover, the gel has three-dimensional porous structure and sufficient mechanical stress resistance, and can be grown in situ in microchannels. Therefore, this immobilization strategy is very suitable for the development of continuous-flow biocatalysis process.