(633g) Invited Talk: Metabolic Engineering of Non-model Yeasts for Production of Chemicals and Fuels
AIChE Annual Meeting
2022
2022 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Systems Biology for Engineering Metabolism
Thursday, November 17, 2022 - 2:18pm to 2:54pm
In the second part, I plan to highlight one technology we recently developed to address the issue of low efficiency for achieving precision genome editing in non-model yeasts. A common technology hurdle for engineering nonconventional species is that majority of them preferentially employ non-homologous end joining (NHEJ) to repair DNA double-strand breaks (DSB), which prohibits precise CRISPR/Cas9 genome editing. Most recently, we identified the shortcomings of the conventional genome editing strategies and developed a new CRISPR platform, named Lowered Indel Nuclease system Enabling Accurate Repair (LINEAR), which enabled precision editing without NHEJ disruption with efficiencies of 67-100% in four industrially relevant yeasts. With NHEJ preserved, we demonstrated its ability to survey genomic landscapes, identifying loci whose spatiotemporal genomic architectures yielded favorable expression dynamics for heterologous pathways.