(169q) Investigating Phase Separation Behavior of Multi-Domain and Intrinsically Disordered Proteins with a Coarse-Grained Model.

Phase separation is a fundamental phenomenon involved in cellular organization, and understanding the behavior of proteins undergoing phase separation is crucial. In this study, we develop a coarse grain model to investigate the phase separation behavior of intrinsically disordered proteins (IDPs) and multi-domain proteins, including folded domains. Our focus is on examining how the presence of folded domains influences the phase separation of these proteins. Our findings contribute to understanding the role of protein structure in phase separation and have implications for cellular processes. This also enables us to capture multi-component systems and understand the compositional control of membrane-less organelles more accurately.