(174ac) Unveiling Plant Perception Mechanisms: Establishment of a Generic Kinase Substratome Identification Platform in Yeast

Kinases are essential enzymes in virtually all life forms that play a fundamental role in the regulation of key processes such as cell signaling, metabolism, cell cycle, and immunity. Understanding the substratome of kinases is crucial for deciphering cellular signaling networks and engineering biological systems. Here, we developed an alternative approach to explore the substratome of plant kinases. We used a model complex, FLS2/BAK1/BIK1, and functionally reconstructed this complex in the model organism Saccharomyces cerevisiae. Through screening the complex against a comprehensive peptide library from the Arabidopsis genome, we identify potential substrates that have not been reported before. Our work demonstrates a complementary strategy for investigating plant perception and facilitates the discovery of new components in the stress response that would otherwise remain hidden using conventional methods.