(178w) The Significance of Coating Implementation on Human Mesenchymal Stem Cells (hMSCs)

Stem cell therapies are medical interventions that use stem cells to replace, regenerate, or repair damaged human tissues, cells, or organs. These therapies aim to harness the unique ability of stem cells to differentiate into various cell types and their capacity for self-renewal, offering potential cures or improvements for a wide range of diseases and conditions. The application of a bioactive surface consisting of collagen and heterologous heparin boosted the capacity of human stem cells to modify the immune system. We evaluated the proliferation and viability of hMSCs on different coatings, both with and without the FGF-2 inhibitor drug AZD454, at a dose of 1 µM in the culture medium. An analysis was undertaken on six various surface types, specifically a control surface (TCPS), a surface composed of 6 layers of HEP/COL, and 6.5 layers of HEP/COL, both with and without the FGF-2 inhibitor drug AZD 4547. Human mesenchymal stem cells (hMSCs) were inoculated onto every surface of a 96-well plate and subsequently cultured for 0, 1, 2, and 3 days. The viability of hMSCs was assessed using PrestoBlue's procedure. Fluorescence detection was performed by introducing 100 μL of PrestoBlue reagent into each well of the cell culture medium, followed by incubation for a period of 3 hours. The proliferation of human mesenchymal stem cells (hMSCs) on COL/HEP coatings can be accomplished by subjecting cells to EdU for a period of 18 hours. After the cells were fixed and made permeable, EdU was detected, DNA was stained, and then the images and data were analyzed. HEP/COL multilayers can improve the viability of hMSCs compared to the control surface. After three days, the results showed that the viability of hMSC was higher than that of TCPS, especially on surfaces with 6.5 heparin and collagen. When human mesenchymal stem cells (hMSCs) are cultured on coatings made from collagen (COL) and heparin (HEP), their proliferation is observed to increase. This phenomenon can be attributed to several factors inherent to the properties of collagen and heparin, which together create a supportive microenvironment for stem cell growth and function. Inhibition studies have indicated that coatings made from heparin (HEP) and collagen (COL) could enhance the proliferation of human mesenchymal stem cells (hMSCs) by activating the Fibroblast Growth Factor-2 (FGF-2) signaling pathway. Heparin binds to FGF-2, protecting it from degradation and facilitating its interaction with FGF-2 receptors on hMSCs.