(504b) Modeling and Performing Multiple Dual Mode Countercurrent Chromatography for Challenging Bioseparations

Valuable targets often require multiple purification steps to attain required purities, increasing capital and operating expenses. Liquid-liquid chromatography (LLC) has been implemented as an orthogonal purification strategy in the pharmaceutical industry and natural product isolations where it has shown great promise as a scalable and resource-efficient separations technique. As separation challenges emerge in the growing sustainable industrial landscape, LLC has gained attention due to the absence of expensive resins, higher loading capacities compared to other chromatographic techniques, and accessibility to a multitude of unique operation modes. In Multiple Dual Mode (MDM), the liquid nature of the stationary phase is advantageously used to resolve overlapping compounds without changing the identity of the stationary or mobile phases. Understanding and implementing MDM can enhance large scale process model development, however, identifying optimal conditions can be a resource- and time-intensive process. In this presentation, we will discuss the further development of the Cell Utilized Partition (CUP) model for predicting liquid-liquid chromatography processes to accommodate MDM experiments. The CUP model prediction and experimental validation will show the efficient separation of lignin-derived aromatic monomers that are typically difficult to separate using distillation. Finally, a sensitivity analysis using the CUP model will provide efficient process optimization and scale-up strategies.