(570b) Enhanced Enzymatic Hydrolysis Conversion Efficiency of Lignocellulosic Biomass Using Hydrothermal Pretreatment

Biomass was utilized in research aimed at generating biofuels, which can serve as substitutes for petroleum or as additives to enhance fuel efficiency. Biomass, in its natural state, has a low enzymatic hydrolysis conversion rate. To address this, pretreatment is necessary to improve the enzymatic hydrolysis rate. Through pretreatment, lignin and sugars can be removed. These removed byproducts can be converted into high-value products. Additionally, glucose derived from biomass can be used as biofuel or for other chemicals. The only solvent used to react with the biomass was water, and no catalysts were employed. The pretreatment was performed at reaction temperatures between 140 and 200 degrees and at various reaction times with batch reactor. The enzymatic saccharification test was conducted with varying amounts of enzymes to determine the change in saccharification efficiency based on enzyme dosage. After that, the enzymatic digestibility of pretreated biomass was compared with the crystallinity of the sample and hemicellulose content, respectively. This study explores an environmentally friendly process using reusable chemicals and achieving a high rate of enzymatic saccharification.