(643f) Understanding the Effects of Pre-Treatment and Extraction Methods on Lipid Profile of Cashew Nut Shell Liquid (CNSL): Non-Targeted Lipidomics Analysis Approach

This novel study delves into the chemical identity of minor components in cashew nutshell liquid (CNSL), a widely used industrial product. Using untargeted lipidomic methods, we analyzed the lipid profile of CNSL, shedding light on the effects of CNSL extraction and cashew nut pre-treatment methods. This research identified key lipid metabolites in CNSL such as Mosinone A, Jasmolone glucoside, and a high content of phosphatidylcholines, which until now had been unknown in samples of the Anacardiciae family. These findings, based on multivariate analysis, also reveal significant differences between CNSL extracted by Soxhlet (SX) and mechanical pressing (P) from CNS pre-treated by roasting (R) or steaming (S). These differences are primarily due to solvent selectivity and lipid thermal degradation. Interestingly, no significant difference was evident between the R and S pre-treatment methods, although pre-treatment had an effect on CNSL when compared with raw nut CNSL. This research expands the understanding of the chemical differences associated with CNSL processing, emphasizing the significant role of pre-treatment on CNS in affecting CNSL, regardless of the method implemented. However, further research is needed to fully identify key lipid metabolites in CNSL samples and their relationship with CNSL processing.