Bioactive Compounds from Extracts of Sarcopoterium Spinosum

Herbal medicines have traditionally been used to prevent and treat various diseases. Sarcopoterium spinosum (S. spinosum), was reported as a potential source of natural compounds to be used in the treatment or prevention of various health problems such as chronic pain, digestive problems, diabetes, and cancer (Hammad, 2018; Smirin et al., 2010). In this study, the antidiabetic effects of the extracts from the aerial and root parts of the S. spinosum were investigated using bioactivity-guided isolation methods. Extraction parameters (percentage of ethanol, extraction time, and solid to liquid ratio) were optimized based on the responses of total antioxidant capacity, total phenol content, and extraction yield, using the Design-Expert program. The extraction conditions for the highest activity of extracts were determined and the isolation of bioactive target compounds was carried using preparative thin-layer chromatography (Prep. TLC). The optimized conditions for extraction of root part were determined as 24 h extraction time, 1:20 solid-liquid ratio, and 90% ethanol. For the extraction of the aerial part, the total antioxidant capacity, total phenol content, and productivity were at their maximum values at extraction conditions of 1:30 solid-liquid ratio, 80% ethanol, and 4-hour. Alpha-glucosidase inhibition tests were performed for the bioactive target compounds obtained from Prep TLC. Acarbose was used as a positive control. Relatively higher inhibition percentages were obtained than acarbose for the two of the bioactive compounds isolated from the extract of the root part.

REFERENCES

Hammad, K.A. (2018). Chemical composition of the methanolic extract from seeds, thorns, and leaves of Sarcopoterium spinosum (L.) (Rosaceae) grown in Al-Tafila, Jordan. Research on Crops, 19, 315–319.

Smirin, P., Taler, D., Abitbol, G., Brutman-Barazani, T., Kerem, Z., Sampson, S.R., Rosenzweig, T. (2010). Sarcopoterium spinosum extract as an antidiabetic agent: In vitro and in vivo study. Journal of Ethnopharmacology, 129, 10–17.