Human Cells-Produced Metallic Nanomaterials: A Look Beyond Biomedical Applications.

In this study, we will clarify ph treated (Ph 13 > Ph5) E.Coli lysate with a single use centrifuge UniFuge®. Day one is to determine feasibility of two application scenarios, The fermenter generates 16 liters of E. Coli . The pcv is approx. 10% after acid treatment. Day one, we will determine shear sensitivity thru pump and UniFuge. We will apply low shear parameters- low g. Day two, we will process 34 liters in 5 trials. Lysate will be PH 5 prior to pump.

Objective- Collect centrate, soluble Mrna for Covid 19 vaccine. This feed stream is reputed to be a shear sensitive process. We will apply low shear parameters to determine the best sequence by NTU, and gel electrophoresis. Longer term analytical results will be performed to check for contamination and potency.

In this study, we will clarify ph treated (Ph 13 > Ph5) E.Coli lysate with a single use centrifuge UniFuge®. Day one is to determine feasibility of two application scenarios, The fermenter generates 16 liters of E. Coli . The pcv is approx. 10% after acid treatment. Day one, we will determine shear sensitivity thru pump and UniFuge. We will apply low shear parameters- low g. Day two, we will process 34 liters in 5 trials. Lysate will be PH 5 prior to pump.