FireProtASR: Fully Automated Pipeline for Resurrecting Stable and Expressible Ancestral Proteins
Synthetic Biology Engineering Evolution Design SEED
2021
2021 Synthetic Biology: Engineering, Evolution & Design (SEED)
Poster Session
Poster Presenters - Accepted
ASR is generally not accessible to scientists outside communities of evolutionary biologists. The purpose of this research is to construct a fully automated pipeline that allows anyone, including those who lack specialist knowledge, to perform ASR, thus reducing the academic barrier to entry. The pipeline was verified against work that was previously done in the lab; on the ancestral sequence reconstruction of haloalkane dehalogenase family5. The pipeline is accessible online through the FireProtASR web server 6 at: https://loschmidt.chemi.muni.cz/fireprotasr/. The server can be used for designing stable, highly expressible and catalytically active enzymes for metabolic pathways, that can be used for the construction of cell factories producing high-value chemicals.
REFERENCES
- Pauling, L., & Zuckerkandl, E., Acta Chemica Scandinavica Supplements 1963, 17, S9âS16.
- Holinski, A., et al., Proteins: Structure, Function, and Bioinformatics 2017, 85.2, 312-321.
- Babkova, P., et al., ChemBioChem 2017, 18, 1448.
- Gaucher, E., et al., Nature 2008, 451, 704
- Musil, M., et al., Briefings in Bioinformatics 2020, bbaa337, 1.
- 6. Khan, R. T., et al., Current Protocols 2021, 1.2, e30.