Small Molecule Detection Via Allosteric Actuation of Strand Displacement Circuits
Synthetic Biology Engineering Evolution Design SEED
2021
2021 Synthetic Biology: Engineering, Evolution & Design (SEED)
Poster Session
Poster Session
Moreover, we harnessed the programmability and modularity of the platform to further enhance the TMSD circuit with the addition of a second signal amplifying cycle for detection of uric acid. Through in silico design of the nucleic acid substrates of the two-cycle system, the biosensorâs response could be fine-tuned in a wider range and detectable within 15 minutes of reaction. We successfully measured uric acid in spiked artificial saliva within the normal human salivary uric acid level and in spiked human serum comparable to a commercial uric acid meter.
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Acknowledgements: Funding support from RGC GRF#16306519, HKPFS from the HKSAR, and Conacyt Mexico is acknowledged.