(184f) Rapid Drug Microadministration toward Neuron Using Electrochemical Micropump for Brain Therapy
AIChE Annual Meeting
2006
2006 Annual Meeting
US - Japan Joint Topical Conference on Medical Engineering, Drug Delivery Systems and Therapeutic Systems
Engineering of Novel Therapeutic Devices
Tuesday, November 14, 2006 - 10:15am to 10:36am
A brain and sensory organs has very complicated system where each neuron plays its individual role. Therefore rapid microinjection toward identified neuron would produce innovation of therapy of brain or sense organs We are interested in micro-administering toward identified neuron. Although micropumps for delivering chemical stimulant are already on practical use, these experiment tools need complicated mechanics and wiring. We then developed a simple micropump which is able to jet out inner solution rapidly by bubbling resulted from electrolysis of water. The micropump consists of a glass capillary (O.D. 1.5mm) with a tip of 10µm in diameter in which platinum wires were inserted as electrodes. It was filled with neurotransmitter solution and sealed by hotglue. We then designed the micropump as an artificial sensory neuron. We used Aplysia's L7 (motor neuron in Abdominal ganglion) as a model cell which is sensitive to glutamate released from sensory neuron. The micropump, which was filled with 10mM glutamate, was placed close (< 10µm) to L7 axonal branches. A potential difference of 3.0V was applied to the electrodes when administering glutamate. Neuronal potential change of L7 was recorded by conventional electrophysiological measurement. Each glutamate administration generated an Excitatory Post Synaptic Potential (EPSP). EPSP decreased gradually with successive administration. This reduction of EPSP is thought to be due to glutamate receptor's desensitization. Then, this pump worked as a substitution of sensory neuron. This micropump could mimic activities and behavior of sensory neuron. We will be able to develop more downsized and integrated micropump because its structure is very simple. The micropump is feasible for potential tool of therapy of brain or sensory organs.
Checkout
This paper has an Extended Abstract file available; you must purchase the conference proceedings to access it.
Do you already own this?
Log In for instructions on accessing this content.
Pricing
Individuals
2006 Annual Meeting
AIChE Pro Members | $150.00 |
AIChE Graduate Student Members | Free |
AIChE Undergraduate Student Members | Free |
AIChE Explorer Members | $225.00 |
Non-Members | $225.00 |
US - Japan Joint Topical Conference on Medical Engineering, Drug Delivery Systems and Therapeutic Systems only
AIChE Pro Members | $100.00 |
Food, Pharmaceutical & Bioengineering Division Members | Free |
AIChE Graduate Student Members | Free |
AIChE Undergraduate Student Members | Free |
AIChE Explorer Members | $150.00 |
Non-Members | $150.00 |