(624c) Development of Rapid Nano-Structured Sensor System for Category B Toxins Detection in Complex Biological and Environmental Samples

Concerns over biosafety and security of environmental system have accelerated the implementation of biosensor technology as a major part of biohazard identification. Traditional multi-step measurement techniques become ineffective due to design and mass transport limitations. Possibility of miniaturization and ability to work with the complex samples (food and environmental matrices) are the major requirements for biosensors. In addition, various nano- and micro-scale physical forces/phenomena determine the effectiveness of the measurement processes in new miniaturized devices. Materials with nano-scale dimensions and high surface-to-volume ratio, such as nano-porous inorganic oxides can be attained by developing structures where the void surface area (pores) is high compared to the amt. of bulk support material. We are presenting a multifunctional biosensor with highly-sensitive nano-patterned transducing elements fabricated by template-synthesis technique. This approach allows developing sensor surfaces with predictable behavior and increasing sensitivity. Successful application of proposed sensor design is demonstrated on real-time detection of Ricin and SEB in various environmental matrices. Detection level of 10 pg/ml achieved in real samples collected in the environment.