Physical Characteristics of Membrane Surface Proteins Resulted from 2,4-Toluene Diisocyanate Addition on Human Adenocarcinoma Cell

As a highly active chemical, 2, 4-toluenediisocyanate (TDI) plays many complicate roles in the stimulation of the abnormal physiological responses through the adductive reaction; including the mechanisms responding for induction of occupational asthma. Previous studies showed the triggering factors onto the receptors may result through an immunological cytokine-interleukin 1, or directly stimulated by TDI. We have found TDI inhibits calcium signaling in human neuroblastoma cells through both nicotinic acetylcholine and muscarinic receptors. Modulators of these ligand gated ion channels act on more than one type of receptor. Similar to toluene TDI may affect ligand-gated ion channel receptor superfamily members also. In this study, we monitored the physical characteristics about changes of the fragments associated with those receptors ligated by TDI conjugation, through mass spectrometric study. Toluene diisocyanate (TDI) is widely used as a chemical intermediate in the production of polyurethane. TDI-induced asthma is related to its disturbance of acetylcholine activity in most affected workers. Our results showed accompany with the suppression of the [Ca2+]c rise induced by the potent nicotinic ligand, epibatidine, in a human epithelial cell line, adenocarcinoma cell culture-Hela cell, there are minute changes in tryptic peptides profile related to TDI.