Recovery of by-Products from Distillers Dried Grains by Sequential Extraction | AIChE

Recovery of by-Products from Distillers Dried Grains by Sequential Extraction

Authors 

Vales Lara, F. M. - Presenter, University of Puerto Rico at Mayaguez
Palomares, M. R. - Presenter, ITESM .Campus Monterrey


A promising approach to improving value for DDG byproduct is for the producer to extract added value from these streams by recovering proteins and oil. The corn DDG was used as a model to find an extraction that optimized the oil and protein recovery by a two-step extraction process in which ethanol was used as primarily solvent. The optimum conditions were; 100% ethanol as solvent (first extraction), 45% ethanol:55% NaOH (second extraction) and a temperature of 25 and 50°C. The sorghum DDG was used to discover which proteins in particular were extracted using SDS- PAGE. To determine protein partitioning behavior an aqueous two-phase (ATP) systems was used. The Bradford method was used to indicate the presence of total protein in the sample. The percentage of oil recovered is higher and protein extraction was lower when the sequential extraction process is executed at 50°C. From the gel and wavelength results it can be concluded that it wasn't possible to identify if the sample has only proteins or if it has other compounds (e.g., phenolic compounds) that will be interfering in the signal.