(212b) Radial Free Flow Electrophoresis for Preparative Separation of Proteins
AIChE Annual Meeting
2007
2007 Annual Meeting
2007 Annual Meeting of the American Electrophoresis Society (AES)
Advances in Electrokinetics and Electrophoresis - Particles and Biomolecules I
Tuesday, November 6, 2007 - 12:55pm to 1:20pm
Electrophoresis is established as a high resolution separation process for protein analysis, but its application for preparative separation in an industrial scale is nowadays restricted due to scale up problems. Different experimental and theoretical studies were devoted to develop alternative operating modes of electrophoresis in order to overcome such limitations. One promising method is free flow electrophoresis (FFE) where the separation is carried out in a flowing liquid film without a matrix medium - as the name ?free flow? implies.
This work deals with the development of FFE in a radial geometry with an accelerated inward flow. The electric field is applied perpendicular to the flow direction. A separation chamber is formed by two parallel ceramic membranes placed between the two electrodes. The distance between the two membranes and thus the separation distance is only a few millimetres. Generally, one major scale-up problem of FFE is the necessity to maintain the hydrodynamic stability of the flow. This requires that the temperature gradient caused by Joule heating must be kept sufficiently low so that the forced flow will remain predominant compared to the buoyancy-driven flow. For the radial FFE, due to the narrow separation chamber and due to intensive cooling at the outer wall of the membranes, the limitation according to Joule heating can be diminished.
Experimental approaches using a model mixture of proteins are carried out to define proper operating conditions according to electrokinetic effects. The performance of the radial FFE in terms of achievable resolution and production capacity are investigated.
Checkout
This paper has an Extended Abstract file available; you must purchase the conference proceedings to access it.
Do you already own this?
Log In for instructions on accessing this content.
Pricing
Individuals
AIChE Pro Members | $150.00 |
AIChE Graduate Student Members | Free |
AIChE Undergraduate Student Members | Free |
AIChE Explorer Members | $225.00 |
Non-Members | $225.00 |