(306b) Purification Of Equine Immunoglobulin G (Igg) From Horse Serum Using An Enhanced Membrane Based Hybrid Bioseparation Technique

Equine immunoglobulin G (IgG) is widely used as antivenom. It is also used anti-rabies and anti tetanus agents. Purification of equine IgG is challenging due to the complexity of serum which contains large amounts of albumin. We discuss an enhanced hybrid bioseparation technique which is based on a standard hybrid bioseparation technique developed earlier in our group for the purification of human IgG. The technique gives high resolution and high throughput by combining ammonium sulphate induced protein precipitation, microfiltration and hydrophobic interaction based membrane adsorption. The enhanced technique overcomes some of the limitations of the standard hybrid bioseparation technique, i.e. antibody dilution and membrane fouling. The equine IgG purity and recovery were both significantly higher than those obtained with currently used commercial techniques