(333n) Identification And Antibody-Therapeutic Targeting Of Antibiotic-Resistant Outer Membrane Proteins In E. Coli

The elucidation of the molecular details of antibiotic resistance will lead to improvements in extending the efficacy of current antimicrobials. Our research focuses on Om proteins in response to antibiotic resistance. This invesitigation was perforemd by proteomic methodologies for the altered Om proteins from survived bacteria in a suddenly strong antibiotic or in sub-MIC of the drug because bacteria my be exposed in the two treatments in their becoming resisitance to the antibiotic. Their shared proteins were further confirmed by Western blotting and gene mutant methods as potential targets for designation of new drugs to inhibit the growth of the antibiotic-resistant bacteria. Moreover, a novel method of specific antibody combating bacterial growth was developed based on these altered Om proteins. Our results suggests that combination therapy involving antibiotics that enhance the expression of an antibody target could be far more effective than either drug alone. Our study give a novel insight into therapy to infection by antibiotic-resistant bacteria. Our study has been extended into a variety of antibiotics including tetracycline, ampicilin, chloramphenicol, chlortetracycline, Streptomycin, kanamycine, nalidixic acid, ceftazidime, ciprofloxacin, balofloxacin, ceftriaxone sodium. *Corresponding author: Dr. Xuanxian Peng This work was sponsored by grants from Guangzhou Key Project 2006Z3-E0251