(402f) Cytotoxicity Of Aggregated Fullerene C60 Particles On Cho And Mdck Cells

The cytotoxicity of fullerene C60 particles on two mammalian cell lines, i.e., the Chinese hamster ovary (CHO) cells and the MDCK (NBL-2) cells, has been investigated. Although innate fullerene particles have a very low solubility in DI water, these particles can be dissolved in the tetrahydrofuran (TFH) solvent at a great value. Further, the dissolved fullerene particles in the TFH solvent could be extracted into a DI water solution at a significantly increased solubility. The formation of fullerene particle aggregates is believed to be the cause of the increased solubility. Results presented here show that, once the concentration of the fullerene aggregates reaches a certain level, the cells start to die. The critical dosage LD50, which is defined as the lowest fullerene concentration, which presented in a given cell culture, results in a 50% cell death within 24 hours, has been determined. Further, both the fullerene concentration and incubation time have negative effect on the cell mortality. These results will help to better understand the side effects of fullerene particles, in mammalian cells.