(48c) Separation Of D-Psicose And D-Fructose From The Binary Mixture Using Simulated Moving Bed Chromatography

D-psicose monosaccharide, a rare sugar used as a valuable pharmaceutical, has been synthesized by converting from D-fructose in an enzymatic reaction. The D-psicose has been studied to isolate from the post-reaction mixture using Simulated Moving Bed technique. The post-reaction mixture of monosaccharides was treated to remove the contaminants such as buffers, proteins, and organic materials by adsorption and ion exchange resins before it was used as feed solution in SMB process. Four columns packed with Dowex 50WX4-Ca2+ 400 mesh ion exchange resin were used for separation of D-psicose and D-fructose in a design of the four-zone SMB with one column per zone. The single-step frontal analysises for binary mixture were carried out to estimate the isotherm parameters for each D-psicose and D-fructose. The operating conditions of SMB process were determined based on the Triangle theory. As the simulation result of SMB process showed that purity and yield of extract product were 99.315% and 99.99%, respectively and those of raffinate product were 99.352% and 99.963%, respectively.