(540a) Cytoskeletal Control of Passive Nuclear Pore Diffusion

Transport across the nuclear envelope is vital for processes such as transcription, signaling and cell regulation. In this study the influence of the cytosketleton on the rate of passive nuclear pore transport was investigated. Using the FRAP technique and micro-injection, the transport rate of green flourescent portein was measured in-situ. A simple mathematical model was used to estimate nuclear permeability from experimental data. Cells were treated with cytoskeletal inhibitors after which the transport rate of green flourscent protein was measured.