(560f) Condition Dependent Regulation Of Lipid Metabolism

Global mapping of transcriptional networks within the framework of network based models can provide valuable insights describing biosynthetic control. We performed multiple high-throughput measurements spanning 8 natural growth conditions to study lipid metabolism in the yeast Saccharomyces cerevisiae in chemostat cultures. Experimental data were mapped onto a reconstructed reaction network centered on lipid biosynthesis and degradation comprising of 104 biochemical reactions, 287 proteins, 106 pathway intermediates, and 284 transcription factor interactions. Our results clarify existing, and reveal new, regulatory motifs underlying lipid biosynthesis. Moreover, they underscore the importance of interrogating cellular systems through the combination of mathematical modeling and experimental biology.