(576d) Micropatterned Fluid Lipid Bilayers Created Using A Continuous Flow Microspotter For Multi-Analyte Assays

There is an increasing interest in the development of microarray based assays for high-throughput analysis and detection of biomolecules such as DNA and proteins. We have developed a new method for creating multianalyte planar supported lipid bilayer (PSLB) arrays using a 3D continuous flow microfluidic system (CFM?). PSLBs are an attractive platform for performing biological assays due to their high resistance to nonspecific biomolecule adsorption and non-fouling nature. PSLBs also serve as good models of biological membranes, allowing a diverse assortment of biological and chemical interactions to be explored in a controlled manner in vitro. The CFM? allows for an addressable microchannel to be formed, in which an array of fluid bilayers can be patterned onto a glass substrate. The system's capability of performing multianalyte assays has potential applications in many fields such as biosensing, drug discovery, proteomics and clinical diagnostics.