(333d) Microfluidic Cell Electroporation Using a Mechanical Valve
AIChE Annual Meeting
2008
2008 Annual Meeting
2008 Annual Meeting of the American Electrophoresis Society (AES)
Biomems and Microfluidics - Novel Applications
Tuesday, November 18, 2008 - 4:18pm to 4:39pm
A microfluidic electroporation technique is demonstrated based on the operation of an elastomeric valve in a polydimethylsiloxane (PDMS) fabricated microchip and a common dc power supply. The pulse needed for permeabilization of the cell membrane is generated by temporarily interrupting the circuit using the valve. The electropermeabilization of suspended and adherent Chinese hamster ovary cells with green DNA dye SYTOX is demonstrated. The technique eliminates the cost and complexity associated with a pulse generator and microfabricated electrodes that are often involved in microscale electroporation devices. It also offers the potential of integrating electroporation as a unit operation in large-scale microfluidic systems with the increasing application of elastomeric valves in these systems.
Checkout
This paper has an Extended Abstract file available; you must purchase the conference proceedings to access it.
Do you already own this?
Log In for instructions on accessing this content.
Pricing
Individuals
AIChE Pro Members | $150.00 |
AIChE Graduate Student Members | Free |
AIChE Undergraduate Student Members | Free |
AIChE Explorer Members | $225.00 |
Non-Members | $225.00 |