(432a) A Scalable 30 Ml Bioreactor for Biopharmaceutical Screening
AIChE Annual Meeting
2008
2008 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
New Approaches In Upstream Bioprocessing: Development and Manufacturing
Wednesday, November 19, 2008 - 8:30am to 8:50am
Glycosylated Biopharmaceutcals are usually produced by suspended mammalian cells (typically Chinese Hamster Ovarium (CHO) cells) cultured in stirred tank bioreactors. However, screening protocols at the lab scale (i.e. to find optimum culture media or initial cell density) mainly rely on Petri dish, bottle or Erlenmeyer flask culture. There is an evident need for small scale fully scalable mammalian cell culture mini-bioreactors for screening processes. Here, we study the mixing performance, mass transfer capabilities, and culture adequacy of 30 mL eccentric stirred tanks. A detailed mixing characterization of the proposed bioreactor is presented. LIF experiments and CFD computations are used to identify operational conditions required for adequate mixing (globally chaotic conditions). Suspended CHO cells are cultured in this novel bioreactor system, and results are compared versus Erlenmeyer flask cultivation. Our results demonstrate good mixing performance, culture convenience, and potential scalability of the proposed screening bioreactor.
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Individuals
2008 Annual Meeting
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AIChE Explorer Members | $225.00 |
Non-Members | $225.00 |
Food, Pharmaceutical & Bioengineering Division only
AIChE Pro Members | $100.00 |
Food, Pharmaceutical & Bioengineering Division Members | Free |
AIChE Graduate Student Members | Free |
AIChE Undergraduate Student Members | Free |
AIChE Explorer Members | $150.00 |
Non-Members | $150.00 |