(682e) Preparation of Bioanalytical Sensors by Incorporating Fluorophore In Patternable Poly(ethylene glycol) Diacrylate-Based Membranes

An oxygen sensor used in cell culture was
constructed by immobilizing fluorophore in PEGDA-based membrane through in
situ photografting technology. This technology allows for the grafting
sensitive membranes, such as analyte-sensitive hydrogels and enzyme immobilized
layers, directly from the negative-tone photoresist SU-8 surface with spatial
control, even within completed microfluidic channels.  The detection
principle of this oxygen sensor was based on the dynamic quenching of the
luminescence of a Ruthenium complex to dissolved oxygen. General principles of
sensor optimization are presented. These include issues such as fluorophore
concentration, thickness control of the membranes and sensitivity enhancement.
The characteristic of the oxygen sensor has also been studied here in details,
such as production stability, long-term stability and response time.  The
particular advantages conferred by the use of in situ photografting
technology are emphasized.  Based on the good results achieved, a
fluorescent glucose biosensor incorporating glucose oxidase and the Ruthenium
complex in PEGDA membrane was also constructed and tested.