(392c) Nanoscale Directed Assembly to Prepare siRNA Containing Liposomes

While liposomal nanoparticles represent a promising class of vectors for siRNA delivery, the attempts with conventional methods have not been successful in controlling the structure of siRNA containing liposomes. Most techniques for liposomal nanoparticle preparation rely on a self assembly process conducted on the macro-scale or in ?bulk,? which is much larger than the nanoparticles themselves and leads to limited control over particle size, structure, composition. We have proof of concept experiments using 1 um features which suggest that carefully controlling the liposome components' interactions create particles with better controlled structure and morphology because it is conducted on the same scale as the particles themselves. This work is being extended to the nanoscale using 200 nm wells in order to further control the particles at the ideal size range. The transfection efficiency of these particles is measured using a breast cancer model using siRNA targeted against the aromatase enzyme.