(416d) Rapid Arraying of Particles and Cells in Three-Dimensional Microfluidic Devices

Direct positioning of particles and cells is used for a variety of applications including molecular detection, self-assembly and tissue or cell engineering. Although there has been a great deal of interest into developing more complex patterning techniques, most suffer from issues in precision, scalability, response times and flexibility. We have developed an arrayed, three-dimensional surface patterned microfluidic device which uses laminar convective flow to position micro-objects on to vacuum actuated holes (drains) into any desired, two-dimensional, computer-programmed pattern. In addition, the device was expanded to independently actuate two independent sets of drains, allowing us to selectively create patterns that have both controlled position and composition. We have used this device to quickly generate anisotropic particles and culture cells from a desired initial pattern and spacing. With rapid, precise and adaptable operation, three-dimensional microfluidic devices can assist novel research and methods by changing an anonymous distribution of particles or cells to an individualized selection.