(687g) Comparison of Polysaccharide Coatings to Limit Protein Adsorption On Polydimethylsiloxane

Polysaccharides can be used to improve the biocompatibility of biomedical devices by providing neutral, heavily hydrated, low surface energy coatings. Polydimethylsiloxane (PDMS) was selected as a substrate for its use in microfluidic and biomedical applications. In this study, we have immobilized several cellulose derivatives and dextran to the surface of polydimethylsiloxane to determine how the surface properties of the polysaccharide layer would affect protein adsorption. The hydrophilicity of the grafted polysaccharides plays a significant role on the morphology of the surface. The dextran layer is tightly bound, and the asparities are narrower and shorter in height. Hydroxyethyl cellulose agglomerates on the surface forming larger 10-30 nm features. Methyl cellulose forms a thick entangled network on the PDMS surface. The influence of roughness on the zeta potential of the PDMS films and adhesion to model hydrophobic and hydrophilic colloids is also measured.