(157f) Combination of Electrospinning and Hydrogel Patterning to Create Microarray System for Dual Sensing

Micropatterning is the important technique in developing miniaturized biosensors and most of micropatterns have been created on various planar substrates. However, the amount of biomolecules that can be immobilized onto a planar surface is limited, affecting sensor performance of relatively weak signal. In order to fully realize potential of protein biochips, proteins need to be immobilized onto surfaces with high density. Among many materials, electrospun nanofibers can provide a large surface area for the attachment of proteins, which can lead to high volumetric protein activity. In this study, we fabricate polymeric nanofibers with a mixture of polystyrene and poly(styrene-alt-maleic anhydride) (PSMA). Proteins can be immobilized onto surfaces of nanofibers without any special modification due to acid anhydride group. And also micropatterned surface was created by poly (ethylene glycol) (PEG) hydrogel microstructures via photolithography on PSMA nanofiber mats. As a result, two different regions are combinatorially fabricated in one substrate. Two different kinds of biomolecules can be detected in each region. Immunoassay system could be prepared by immobilizing mouse immunoglobulin G (IgG) on PSMA nanofibers and different concentration of fluorescence labeled rabbit anti-mouse IgG was used. Glucose oxidase (GOX) and Peroxidase are entrapped in hydrogel region. The GOX-catalyzed oxidation reaction was investigated with Amplex Red and different concentrations of glucose.