(372j) Temperature Distribution in Electrochromatography with An Oscillatory Transverse Electric Field

A three-compartment column of ion-exchange electrochromatography with an oscillatory electric field perpendicular to mobile-phase flow driven by pressure (pIEEC) was verified for its high throughput purification of proteins¹. Electroosmotic flow was proved to be major contributor to its mass transfer acceleration². By modeling work, protein distribution in adsorbents was found to present excursion along the electric field direction. The electro-kinetic convection in porous particle was predicted as exponentially decreased function of protein adsorption amount³. So that, at the beginning of the pIEEC, intraparticle convection caused by the electric field contributed more to the enhancement of dynamic binding capacity. For utilization of the new design, an experimental system for in-column temperature measurements was constructed, and the dynamic processes of the in-column temperature in electrochromatography were examined. With the experimental system, the effect of electric current strength and mobile-phase ionic strength on the in-column temperature was investigated. Then, a heat transfer model for the pIEEC was established for the dynamic process modeling. The mathematical model concerns force and free convection heat transfer, as well as Darcy modified wall effect in packed bed column. It was confirmed that the model was in good agreement with the measurements. By the model calculations, we could also obtain the in-column voltage distribution, effective voltage applied to the central compartment and the efficiency of energy consumption. Moreover, the model was used for scale-up analysis and for investigation of temperature distribution under various conditions.

1. Journal of Separation Science, 2006, 29, 2383; 2. Separation and Purification Technology, 2009, 68, 109-113 3. Electrophoresis, 2010, 31, 944-951