(67b) Metabolomics Reveals the Relationship Between Ketogulonigenium Vulgare and Bacillus Megaterium
AIChE Annual Meeting
2010
2010 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Systems and Synthetic Biology of Interacting Microorganisms
Monday, November 8, 2010 - 8:50am to 9:10am
Ketogulonigenium vulgare and Bacillus megaterium were found to form a natural dual ecosystem and has been utilized to produce 2-keto-L-gulonic acid (2-KLG), precursor of vitamin C, for over 30 years. Understanding the metabolic interaction is critical to improving the vitamin C production. In this work, a comparative metabolomics analysis strategy was used to investigate the profiles of the molecular compositions of metabolites both in vivo and in vitro so as to elucidate the changes in metabolic interaction of this ecosystem. Significant changes in metabolites related to chemitaxis, central carbon metabolism and sporulation of Bacillus were explored.