(85e) Sustained and Localized Delivery Systems with Virus-Polymer Hybrid

The design and evaluation of drug/gene delivery systems for sustained and localized release profiles to target sites of interest is desirable for the prolonged treatment and the precise introduction of therapeutic products, alleviating the concern of an off-targeting effect. Biomaterial scaffolds can provide an environment that facilitate tissue/cell adhesion (mechanical support) and allows drug/gene delivery vectors to be employed for the sustained release in a spatially controlled manner. In this study, we have investigated a strategy based on a porous scaffold of PLGA polymer associated with other immunostimulatory molecules (i.e. cytokines) and foreign substances (antigen) or viral vectors such as adenovirus and lentivirus to recruit host dendritic cells and further educate them in situ for developing immune defenses. In two weeks after implantation, the recruitment of dendritic cells (CD11c+) was observed in the implant. Antigen-specific immune responses with different combination of cytokines and antigens or viral vectors will be addressed in more detail.