(9d) Endothelial Progenitor Cell Rolling and Capture On Biomaterial Surfaces
AIChE Annual Meeting
2010
2010 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Biomaterial-Cell Interactions in Tissue Engineering
Monday, November 8, 2010 - 9:30am to 9:50am
Endothelial
progenitor cells (EPCs) have the potential to become a reliable source of
autologous cells for endothelialization of intravascular devices and
vascularization of tissue engineered constructs. In this project, we have
characterized the rolling and capture of EPCs on different protein-coated
surfaces using a parallel plate flow chamber. These results will be applied in
the design of future biomaterial surfaces to enhance endothelialization and
improve EPC strength of adhesion under shear stress. EPCs are
blood-derived cells and little is currently known about their response to shear
stress. For these studies, umbilical cord blood outgrowth endothelial
colony forming cells (ECFCs) were used. ECFCs were first expanded in
culture. To assess ECFC ability to interact with our material coatings
under shear stress, ECFCs were dissociated and suspended in flow media. Using a
Glycotech parallel plate flow chamber, the ECFC cell suspension was sheared
over collagen-coated tissue culture polystyrene (TCPS), gelatin-coated TCPS and
CellBIND surfaces with shear rates of 40s-1, 80s-1, and
120s-1. Tethering of ECFCs was shown to relate to shear rates and
adhesion material surface. Transient adhesion of ECFCs occurs more frequently
on collagen-coated TCPS than the other two adhesion material surfaces.
Migration of ECFCs on these surfaces under both static and shear conditions was
also studied. We have also studied the adhesion and
spreading of EPCs and polyethylene glycol diacrylate (PEG-DA) hydrogels with
covalently coupled Arg-Gly-Asp-Ser
(RGDS). Future
studies will investigate the role of specific integrin binding sites in ECFC
rolling and capture under shear stress. Capture of rolling ECFCs could be
maximized by engineering biomaterials to incorporate the appropriate binding
ligands. Our results provide a better understanding of ECFCs-material
interactions under physiological shear stress and will aid in the design of
materials for stent coating and vascular grafts as well as for other
intravascular applications.
progenitor cells (EPCs) have the potential to become a reliable source of
autologous cells for endothelialization of intravascular devices and
vascularization of tissue engineered constructs. In this project, we have
characterized the rolling and capture of EPCs on different protein-coated
surfaces using a parallel plate flow chamber. These results will be applied in
the design of future biomaterial surfaces to enhance endothelialization and
improve EPC strength of adhesion under shear stress. EPCs are
blood-derived cells and little is currently known about their response to shear
stress. For these studies, umbilical cord blood outgrowth endothelial
colony forming cells (ECFCs) were used. ECFCs were first expanded in
culture. To assess ECFC ability to interact with our material coatings
under shear stress, ECFCs were dissociated and suspended in flow media. Using a
Glycotech parallel plate flow chamber, the ECFC cell suspension was sheared
over collagen-coated tissue culture polystyrene (TCPS), gelatin-coated TCPS and
CellBIND surfaces with shear rates of 40s-1, 80s-1, and
120s-1. Tethering of ECFCs was shown to relate to shear rates and
adhesion material surface. Transient adhesion of ECFCs occurs more frequently
on collagen-coated TCPS than the other two adhesion material surfaces.
Migration of ECFCs on these surfaces under both static and shear conditions was
also studied. We have also studied the adhesion and
spreading of EPCs and polyethylene glycol diacrylate (PEG-DA) hydrogels with
covalently coupled Arg-Gly-Asp-Ser
(RGDS). Future
studies will investigate the role of specific integrin binding sites in ECFC
rolling and capture under shear stress. Capture of rolling ECFCs could be
maximized by engineering biomaterials to incorporate the appropriate binding
ligands. Our results provide a better understanding of ECFCs-material
interactions under physiological shear stress and will aid in the design of
materials for stent coating and vascular grafts as well as for other
intravascular applications.