(501a) Peptoid Based Slide Coatings for Disease Detection Via ELISA Microarray Analysis

Pursuit of an efficient, non-invasive, and economical tool for the early detection of diseases has been the passion of many researchers. A large factor in the ability to create one of these tools is the platform on which to build a reliable test. The use of peptoid-based slide coatings for sandwich enzyme-linked immunosorbent assay (ELISA) microarrays is a promising start to detecting small concentrations of protein biomarkers in biological solutions. Peptoids are believed to have the structure necessary to achieve the critical properties of specific binding sites, high surface area, retention of antibody structure, long shelf-life, and low non-specific protein binding. Preliminary results of recently designed peptoids have shown to form microspheres as surface structures which gives high probability for peptoids achieving these necessary surface properties. The peptoid coated slides will be compared to the currently used lysine-based slides which have shown good performance in past ELISA microarray studies. The comparisons between the slides will be based on spot background, slide noise, spot size and morphology, consistency between slides, and lower limit of detection. We believe peptoid-based slide coatings will create the platform needed to build a reliable test for the early detection of cancer, leading to an increased survival rate.