(625p) Antibacterial Activity of Doxycycline Loaded Chitosan/Poly(L-lactic acid)/Polycaprolactone Blend for Wound Dressing Device

Wasinee Boonkong^a, Amorn Petsom^b, and Nuttha Thongchul^b

^aProgram in Petrochemistry , Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

^bInstitute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330 Thailand

Keywords : Chitosan, , Poly(L-lactic acid), doxycycline, Antibacterial, and wound dressing

Chitosan and polylactic acid (PLA) are the renewable, compostable, and biocompatible polymers that provide many useful applications in various areas while polycaprolactone (PCL) that is used to improve processing characteristics of resulting plastics when blended or copolymerized with other polymers. Recent works report the antibacterial activity of doxycycline loaded Chitosan/Poly(L-lactic acid)/Polycaprolactone blend film against Staphylococcus aureus and Escherichia coli, as model Gram-positive and Gram-negative bacteria, respectively. In this work, we synthesized low molecular weight PLA via direct polycondensation. Low molecular weight PLA was then blended with polycaprolactone to enhance impact resistance. Later, the PLA/PCL blend was mixed with chitosan solution and then casted into the film using solvent evaporation technique. Glycerol was added in order to promote casting. The blended film was studied as a model device to deliver drug to targeted human organ which in this case was the skin with opened wound. The model drug used in this study, doxycycline, was loaded into the blend before casting. Film properties and the delivery rate of doxycycline were investigated. The mechanical properties including swelling, tensile and T-peel tests were also observed. The loaded chitosan/Poly(L-lactic acid)/Polycaprolactone film delivery study gave a high % release rate which possibly reached 100% loaded. In vitro an antibacterial test of the virgin chitosan film was demonstrated its ability to inhibit common bacterial growth (S. aureus and E. coli) for a prolonged duration. The blended film without doxycycline was unable to inhibit both bacteria while the presence of doxycycline in the film led to the highest degree of inhibition in both bacterial cultures.